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| Missio,Robson Fernando; Caixeta,Eveline Teixeira; Zambolim,Eunize Maciel; Pena,Guilherme Ferreira; Ribeiro,Ana Paula; Zambolim,Laércio; Pereira,Antônio Alves; Sakiyama,Ney Sussumu. |
| EST-SSR markers were used to investigate the genetic diversity among and within coffee populations, to explore the possibility of their use for fingerprinting of cultivars and to assist breeding programs. Seventeen markers, developed from ESTs (Expressed Sequence Tags) from the Brazilian Coffee Genome Project, were used. All markers showed polymorphism among the genotypes assessed. The average number of allele per primer was 5.1. The highest polymorphisms were found within C. canephora (88.2%) and rust-resistant varieties (35.3%). About 29.4% of the markers differentiated C. arabica from Híbrido de Timor; it was also possible to identify those closest and farthest from C. arabica . The analysis of population-grouped genotypes revealed a 64.0% genetic... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: DNA markers; Coffea sp.; UPGMA; Amova; Microsatellite markers. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0006-87052009000300003 |
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| Bordin,Ivan; Missio,Robson Fernando; Casimiro,Evandro Luiz Nogarolli; Castro,Ana Maria Conte e; Fey,Emerson. |
| O trabalho teve como objetivo avaliar o desenvolvimento de mudas de pupunheira (Bactris gasipaes H.B.K.) cultivadas em amostras de um Latossolo Vermelho eutroférrico de textura argilosa compactado artificialmente em subsuperfície. As amostras de solo que foram compactadas a quatro níveis de densidade (1,0; 1,13; 1,28 e 1,44kg dm-3) estavam nas camadas intermediárias de 3,5cm de vasos de PVC de 20cm de diâmetro, que constavam de outras camadas superiores de 15cm e inferiores de 10cm. As avaliações foram realizadas em quatro épocas (4, 6, 8 e 10 meses). O aumento dos níveis de densidade do solo não influenciou no desenvolvimento da parte aérea das mudas de pupunheira. Independentemente da época estudada, os níveis de densidade do Latossolo argiloso não... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Sistema radicular; Bactris gasipaes; Densidade do solo. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782006000100046 |
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| Ceresini,Paulo Cezar; Silva,Cristina Lacerda Soares Petrarolha; Missio,Robson Fernando; Souza,Elaine Costa; Fischer,Carlos Norberto; Guillherme,Ivan Rizzo; Gregorio,Ivo; Silva,Eloiza Helena Tajara da; Cicarelli,Regina Maria Barreto; Silva,Marco Túlio Alves da; Garcia,José Fernando; Avelar,Gustavo Arbex; Porto Neto,Laercio Ribeiro; Marçon,André Ricardo; Bacci Junior,Maurício; Marini,Danyelle Cristine. |
| The main goal of our research was to search for SSRs in the Eucalyptus EST FORESTs database (using a software for mining SSR-motifs). With this objective, we created a database for cataloging Eucalyptus EST-derived SSRs, and developed a bioinformatics tool, named Satellyptus, for finding and analyzing microsatellites in the Eucalyptus EST database. The search for microsatellites in the FORESTs database containing 71,115 Eucalyptus EST sequences (52.09 Mb) revealed 20,530 SSRs in 15,621 ESTs. The SSR abundance detected on the Eucalyptus ESTs database (29% or one microsatellite every four sequences) is considered very high for plants. Amongst the categories of SSR motifs, the dimeric (37%) and trimeric ones (33%) predominated. The AG/CT motif was the most... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bioinformatics tool; Genetic markers; Gene ontology; Small sequence repeats; SSR. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000400014 |
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